The aim of this study was to investigate the effects of supplementing with 400 mg of lemon verbena extract (Recoverben®) on muscle strength and recovery in healthy, moderately active adults. We found that consumption of lemon verbena significantly attenuated loss of muscle strength compared to placebo. Muscle strength loss is considered a reliable and valid functional marker for assessing muscle damage . Therefore, our preliminary findings suggest that lemon verbena may reduce exercise-induced muscle damage.
Muscle strength was reduced by 21% in placebo group, which is within the expected range of 20–50% and recovery was not completed until two days post exercise. It is generally accepted that two to seven days are necessary for full recovery following exercise induced muscle damage . Compared to placebo, lemon verbena extract significantly (p = 0.0311) buffered strength loss after exercise. MVC in the lemon verbena group was reduced by 11%, which is defined as mild muscle damage . Furthermore, complete recovery was reached after 48 h. Based on these findings, lemon verbena appears to not only speed recovery, but also reduce fatigue directly after exercise.
These results were reflected by findings for perceived muscle soreness. Movement induced pain, which estimated actual perceived pain showed discrimination between study groups with slight superiority of lemon verbena extract by trend. The less pronounced muscle damage, seen by significantly less reduction of MVC, seems to be reflected by less perceived pain under lemon verbena extract if compared to placebo. Maximum of muscle soreness was reported 48 h after exercise, fitting to the general knowledge that muscle soreness peaks 24 h or 48 h after damaging exercise . The extent of muscle soreness was medium for both groups, supporting that the exercise protocol caused mild to moderate muscle damage. After 96 h, subjects were, on average, not completely painless, even if muscle strength at that time was already recovered in this group. However, the same phenomenon has already been observed by others [9, 33].
Increasing concentration of CK in the blood is an indication of muscle damage, being frequently used in sports nutrition studies [9, 35]. The time course of CK increase peaked at 24 h after exercise, which is comparable to findings reported in literature [9, 35]. Exercise-induced increases in CK are known to exhibit high interindividual variability, with some people showing large increases (responders) and others showing only moderate increases (non-responders) . In our study, high levels of interindividual variation in CK concentrations were present, which could explain why we failed to observe a significant between-group difference despite other markers of muscle damage, such as MVC, favoring the lemon verbena group.
Many research studies have shown that supplementation with dietary polyphenols has the potential to positively influence symptoms of exercise-induced muscle damage [2, 5, 8–10, 41, 19]. However, underlying processes are still unclear and it is not sure if antioxidative effects are the primarily mechanisms . Furthermore, the benefit of reducing oxidative stress has been discussed diversely [41, 42]. Increased of oxidative stress can lead to progressive cell damage and decline in physical function [42, 43]. However, ROS act as biological stimuli in cellular processes of adaption to training [41, 42] and cells can adapt to repetitive increases of ROS by improving antioxidant capacity [44, 45]. During the current study, glutathione peroxidase in plasma was selected as a parameter to supply information about antioxidative capacity. Our results indicate an activation of the antioxidative defense under lemon verbena extract by up-regulating GPxP shortly after exercise. In contrast to this, baseline GPxP was not increased by supplementation with lemon verbena extract. Therefore, it appears, that supplementation with lemon verbena extract strengthens the antioxidative defense system and enables effective counteraction of oxidative stress, but only if needed. Both groups experienced significant exercise-induced increases in IL-6 without significant difference between one another. Some evidence suggests that changes in IL-6 depend in part on exercise intensity and duration [1, 46, 40]. It is possible that the exhaustive exercise protocol used in our study was not intense and/or long enough to elicit meaningful changes in IL-6 that could have been effected by lemon verbena supplementation. Similar results were found in other human studies investigating natural ingredients for effects of muscle strength and muscle damage, such as ashwagandha extract , curcumin , pomegranate extract , and blueberry . These natural ingredients are high in polyphenols, a trait shared by lemon verbena. It has been proposed, that polyphenols could be useful to prevent muscle damage or improve recovery . The major biological functions of polyphenols are as antioxidants and anti-inflammatory agents. Enhanced production of vasodilation factors and the inhibition of synthesis of vasoconstrictors have also been shown . These could be additionally beneficial by improving tissue oxygen supply and removal of metabolic waste products. The proprietary lemon verbena extract (Recoverben®) investigated in the current study has shown anti-inflammatory effects  and is characterized by a high polyphenol content and high ORAC level. Therefore, the observed reduction in muscle strength loss and indicated accelerated recovery in the present study might be explained by lemon verbenas’ constituents and their ability to prevent or reduce inflammatory processes or reduce oxidative stress.